Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Muscle stem cells contribute to long‐term tissue repletion following surgical sepsis

doi: 10.1002/jcsm.13214

Figure Lengend Snippet: Satellite cell reduction reduces muscle regeneration capacity in cecal ligation and puncture/daily chronic stress (CLP/DCS) mice. ( A ) Timeline representing male Pax7‐DTA mice treated for five consecutive daily injections of tamoxifen or vehicle, 15 days before surgery. Percentage change from baseline of ( B ) lean mass. ( C ) Immunofluorescence images (top) and quantification of minimum Feret diameter (bottom) of tibialis anterior (TA) cross sections from either vehicle or tamoxifen control and CLP/DCS mice. Green = laminin, Blue = DAPI. Images are ×20 with scale bar = 200 μm. ( D ) Pax7 staining (top) and quantification of Pax7‐positive nuclei per myofibre (bottom) of tibialis anterior (TA) cross‐sections from vehicle or tamoxifen control and CLP/DCS mice. Purple = haematoxylin (nuclei), Red = Pax7. Images are ×40 with scale bar = 50 μm. All images are representative. B = Two‐way ANOVA with Bonferroni multiple comparisons (BMC) where *significant vs. control vehicle and control tamoxifen, ^significant vs. control tamoxifen, and % significant vs. control vehicle, P < 0.05. C = nonlinear fit pairwise comparisons. D = One‐way ANOVA with BMC where significance = * P < 0.05, ** P < 0.01, *** P < 0.001. See Tables S4 and S5 for all P values from all comparisons in ( B ) and ( C ), respectively. N = 3–7. Each n = one mouse.

Article Snippet: Permeabilization was performed using 0.5% Triton X‐100 (Sigma) in PBS for 5 min at room temperature (RT), then washed ×2 in immunofluorescence buffer (IFB: 3% bovine serum albumin [BSA, GoldBio], 0.2% Triton X‐100 (Sigma), 0.2% Tween‐20 [Sigma], in PBS).

Techniques: Ligation, Immunofluorescence, Control, Staining

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Muscle stem cells contribute to long‐term tissue repletion following surgical sepsis

doi: 10.1002/jcsm.13214

Figure Lengend Snippet: Characterization of muscle loss and recovery in CLP/DCS mice. ( A ) Combined cecal ligation and puncture (CLP)/daily chronic stress (DCS) Kaplan–Meier survival curve versus control mice to experiment endpoint. ( B ) Serum levels of mouse C‐reactive protein (CRP) and aspartate aminotransferase (AST) activity. ( C ) Control and CLP/DCS percentage change from baseline in lean mass. Data from mouse MRI on Day 3 or 4 are represented on lean mass graph as Day 3. ( D ) Harvested wet weights, as change in percentage of control, of tibialis anterior (TA, top) and gastrocnemius (GR, bottom) normalized to baseline body weight, respectively, in control and CLP/DCS Days 4, 8 and 28. ( E ) Immunofluorescence images (top) and quantification of minimum Feret diameter (bottom) of tibialis anterior (TA) cross‐sections from control, Days 4, 8 and 28 CLP/DCS mice. Green = laminin, Blue = DAPI. Images are representative and ×20 with scale bar = 200 μm. See Table S1 for all P value comparisons. ( F ) Immunofluorescence images (top) and quantification (bottom) of tibialis anterior (TA) myofibre typing from control, Days 4, 8 and 28 CLP/DCS mice. Red = type IIb, Green = type IIa, Unstained = type IIx, White = laminin. Images are representative and ×20 with scale bar = 100 μm. Data from muscle mass/minimum Feret diameter/myofibre typing were harvested on Day 27 or 28 and are labelled as Day 28. B = log‐rank test. C , E , G = One‐way ANOVA with Bonferroni multiple comparisons (BMC). D = Two‐way ANOVA with BMC. F = nonlinear fit pairwise comparisons. Significance: * P < 0.05, **/ & P < 0.01, ***/ # P < 0.001. ( B ) n = 12–13. ( C–D ) n = 16–45. ( E ) n = 11–14. ( F ) n = 5. ( G ) n = 10–14. Each n = one mouse.

Article Snippet: Permeabilization was performed using 0.5% Triton X‐100 (Sigma) in PBS for 5 min at room temperature (RT), then washed ×2 in immunofluorescence buffer (IFB: 3% bovine serum albumin [BSA, GoldBio], 0.2% Triton X‐100 (Sigma), 0.2% Tween‐20 [Sigma], in PBS).

Techniques: Ligation, Control, Activity Assay, Immunofluorescence

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Muscle stem cells contribute to long‐term tissue repletion following surgical sepsis

doi: 10.1002/jcsm.13214

Figure Lengend Snippet: Muscle recovery deficit in post‐sepsis mice with secondary muscle injury. ( A ) Timeline depicting experimental setup of second insult to muscle in control and cecal ligation and puncture/daily chronic stress (CLP/DCS) mice. ( B ) Immunofluorescence images (top) and minimum Feret diameter quantification (bottom) of tibialis anterior (TA) cross‐sections in control or CLP/DCS mice that received vehicle or BaCl 2 (injured) injections. Green = laminin, Blue = DAPI. Images are ×20 with scale bar = 200 μm. ( C ) Pax7 staining (top) and quantification of Pax7‐postive nuclei per myofibre of TA cross‐sections from vehicle or injured control and CLP/DCS mice. Purple = haematoxylin (nuclei), Red = Pax7. Images are ×40 with scale bar = 50 μm. All images are representative. B = nonlinear fit pairwise comparisons, see Table S6 for all P values from all comparisons. C = One‐way ANOVA with Bonferroni multiple comparisons (BMC). Significance is * P < 0.05, ** P < 0.01, *** P < 0.001. N = 3–6. Each n = one mouse.

Article Snippet: Permeabilization was performed using 0.5% Triton X‐100 (Sigma) in PBS for 5 min at room temperature (RT), then washed ×2 in immunofluorescence buffer (IFB: 3% bovine serum albumin [BSA, GoldBio], 0.2% Triton X‐100 (Sigma), 0.2% Tween‐20 [Sigma], in PBS).

Techniques: Control, Ligation, Immunofluorescence, Staining

Journal: Clinical Kidney Journal

Article Title: Renal diseases secondary to interferon-β treatment: a multicentre clinico-pathological study and systematic literature review

doi: 10.1093/ckj/sfab114

Figure Lengend Snippet: Demographic, clinical and laboratory characteristics of the 18 patients with IFN-β-associated nephropathy

Article Snippet: Four patients were treated with IFN-β1b (all with Betaferon, Bayer AG, Leverkusen, Germany).

Techniques: Biomarker Discovery, Tandem Mass Spectroscopy

Journal: Clinical Kidney Journal

Article Title: Renal diseases secondary to interferon-β treatment: a multicentre clinico-pathological study and systematic literature review

doi: 10.1093/ckj/sfab114

Figure Lengend Snippet: Comparison of the type and dosage of IFN-β in patients with TMA and in all patients treated in France

Article Snippet: Four patients were treated with IFN-β1b (all with Betaferon, Bayer AG, Leverkusen, Germany).

Techniques: Comparison, Injection

Journal: Journal of Neurology, Neurosurgery, and Psychiatry

Article Title: Pregnancy outcomes in patients exposed to interferon beta-1b

doi: 10.1136/jnnp-2014-308113

Figure Lengend Snippet: Birth defects reported in pharmacovigilance database

Article Snippet: The objective of the present analysis was to review pregnancy outcomes in patients who were exposed to interferon beta-1b (Betaferon/Betaseron; Bayer HealthCare Pharmaceuticals) during pregnancy.

Techniques: Medications, Infection, Capsules

Journal: Neurology and Therapy

Article Title: A Prospective, Observational, Multicenter Study Assessing Adherence to Interferon Beta-1b Therapy and Patient Satisfaction Using the BETACONNECT Auto-Injector

doi: 10.1007/s40120-022-00323-1

Figure Lengend Snippet: Study inclusion and exclusion criteria

Article Snippet: Interferon beta-1b (Betaseron, Bayer US LLC, Whippany, NJ, USA) is a type of DMT for patients with CIS or RRMS.

Techniques: Biomarker Discovery

Journal: Neurology and Therapy

Article Title: A Prospective, Observational, Multicenter Study Assessing Adherence to Interferon Beta-1b Therapy and Patient Satisfaction Using the BETACONNECT Auto-Injector

doi: 10.1007/s40120-022-00323-1

Figure Lengend Snippet: Patient demographics

Article Snippet: Interferon beta-1b (Betaseron, Bayer US LLC, Whippany, NJ, USA) is a type of DMT for patients with CIS or RRMS.

Techniques:

Journal: Neurology and Therapy

Article Title: A Prospective, Observational, Multicenter Study Assessing Adherence to Interferon Beta-1b Therapy and Patient Satisfaction Using the BETACONNECT Auto-Injector

doi: 10.1007/s40120-022-00323-1

Figure Lengend Snippet: Adherence rates among patients

Article Snippet: Interferon beta-1b (Betaseron, Bayer US LLC, Whippany, NJ, USA) is a type of DMT for patients with CIS or RRMS.

Techniques:

Journal: Neurology and Therapy

Article Title: A Prospective, Observational, Multicenter Study Assessing Adherence to Interferon Beta-1b Therapy and Patient Satisfaction Using the BETACONNECT Auto-Injector

doi: 10.1007/s40120-022-00323-1

Figure Lengend Snippet:

Article Snippet: Interferon beta-1b (Betaseron, Bayer US LLC, Whippany, NJ, USA) is a type of DMT for patients with CIS or RRMS.

Techniques:

Journal: Brain and Behavior

Article Title: An update on immunopathogenesis, diagnosis, and treatment of multiple sclerosis

doi: 10.1002/brb3.362

Figure Lengend Snippet: Approved therapies for multiple sclerosis

Article Snippet: Beta-interferon-1b (Extavia®, Bayer HealthCare Pharmaceuticals Inc., Montville, NJ) , 250 μg , SC , Every other day.

Techniques: Injection, Blocking Assay, Virus, Infection, Membrane